Biochemical analysis of vitellogenin from rainbow trout (Salmo gairdneri) : fatty acid composition of phospholipids

Biochemical analysis of vitellogenin from rainbow trout (Salmo gairdneri): fatty acid composition of phospholipids.

Vitellogenin was obtained from three year-old vitellogenic trout. Two procedures of isolation were compared: dialysis against distilled water and ultracentrifugation in the density interval 1.21-1.28 g/ml. Similar patterns were observed by gel filtration and electrophoresis for both preparations of vitellogenin, indicating that electric charge and molecular weight were not modified by either pr...

Molecular species composition of the major phospholipids in brain and retina from rainbow trout (Salmo gairdneri). Occurrence of high levels of di-(n-3)polyunsaturated fatty acid species.

The molecular-species compositions of the diacyl classes of the major phospholipids from the brain and retina of rainbow trout (Salmo gairdneri) were determined. A total of 46 possible species was identified. Didocosahexaenoyl species were major components of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylserine (PS) from retina, comprising 14.1, 41.3 and 28.3% of the r...

Toxicokinetics of Fenvalerate in Rainbow Trout (Salmo Gairdneri)

An in vivo rainbow trout (Salmo gairdneri) preparation was used to evaluate the gill uptake and toxicokinetics of [3H]fenvalerate ([R,S]-α-cyano-3-phenoxybenzyl [R,S]-2-[4-chloro-phenyl]-3-methylbutyrate), a synthetic pyrethroid insecticide. Fish were exposed to technical-grade fenvalerate (0.28 or 23 ng/L) or an emulsifiable-concentrate formulation (16 ng/L) for 36 to 48 h. No significant effe...

Nocardial Infection in Hatchery-reared Fingerling Rainbow Trout (salmo Gairdneri).

The effect of dietary composition and of insulin on gluconeogenesis in rainbow trout (Salmo gairdneri).

1. The activities at 15" of three gluconeogenic enzymes, D-fructose-l,6-diphosphate, 1-phosphohydrolase (EC 3.1.3.1 l), pyruvate carboxylase (EC 6.4.1.1) and phosphoenolpyruvate carboxykinase (4.1.1.32), were determined in liver, kidney, gill and muscle of rainbow trout (Sulmo guirdneri) given a commercial diet. The results indicated that liver and kidney are the main sites of gluconeogenesis. ...